PROTOCOLS

Baculovirus Production for Recombinant Proteins - http://axon.med.harvard.edu/~cepko/protocol/mike/B1.html

Baculovirus Production for Recombinant Proteins. Protocol is a modified version of the pharmingen Baculogold kit. Brian Dynlacht. Mike A. Dyer Lab. - [Read more Baculovirus Production for Recombinant Proteins]

Expression of F-tryptophan labelled protein - http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/fluoexp.html

Expression of F-tryptophan labelled protein. Protocol can be adapted for Fluoro-phenylalanine or Fluro-tyrosine labelling. Transformation of competent E. coli cells for expression with plasmid. Dr. Chen, Dept. of Biochem. & Mol. Biology, University College, London. - [Read more Expression of F-tryptophan labelled protein]

Expression of protein in minimal media - http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/protexp.html
Expression of protein in minimal media using E. coli cells for expression. Dr. Chen, Biochem. & Mol. Biology, University College. London - [Read more Expression of protein in minimal media]

High Yield Protein Production from Pichia pastoris Yeast PDF - http://www.nbsc.com/files/papers/abl_pichia.pdf
High Yield Protein Production from Pichia pastoris Yeast PDF. Julia Cino, PhD - [Read more High Yield Protein Production from Pichia pastoris Yeast PDF]

K. lactis Protein Expression Kit PDF - http://www.neb.com/nebecomm/ManualFiles/manualE1000.pdf
K. lactis Protein Expression Kit. Recombinant Protein Expression in Yeast. NEB Biolabs - [Read more K. lactis Protein Expression Kit PDF]

KickStart™ Protein Expression Kit PDF - http://www.dualsystems.com/pdf/KickStart/P01006_KickStart.pdf
KickStart™ Protein Expression Kit PDF. Dual Systems Biotech - [Read more KickStart™ Protein Expression Kit PDF]

Protein Expression and Purification Flowchart - http://www.embl.de/ExternalInfo/protein_unit/draft_frames/frame_flowchart_ext.htm
Protein Expression and Purification Flowchart. EMBL - [Read more Protein Expression and Purification Flowchart]

Protein Expression Protocols and Applications Guide PDF - http://www.promega.com/paguide/chap5.pdf
Protein Expression Protocols and Applications Guide PDF. Promega. In vitro translation systems in Prokaryotes and Eukaryotes. Promega. - [Read more Protein Expression Protocols and Applications Guide PDF]

Protein Expression Techniques Advantages and Disadvantages - http://www.molecularstation.com/word_blog/protein-expression-techniques/
Protein Expression Techniques Advantages and Disadvantages. Molecular Station. - [Read more Protein Expression Techniques Advantages and Disadvantages]




Non-denaturing Cell Lysis Buffer - http://www.chemicon.com/techsupp/celllysis.asp
Non-denaturing Cell Lysis Buffer. Chemicon. - [Read more Non-denaturing Cell Lysis Buffer]

Western Blotting and Recipies - http://www.ualberta.ca/PERINATAL/facilities/pdf/Western--Protein%20preparation.pdf
Western Blotting and Recipies. Lysis Buffer. - [Read more Western Blotting and Recipies]